Journal: Animals : an Open Access Journal from MDPI
Article Title: Screening and Identification of Differential Ovarian Proteins before and after Induced Ovulation via Seminal Plasma in Bactrian Camels
doi: 10.3390/ani11123512
Figure Lengend Snippet: Validation of differentially expressed proteins (DEPs). ( A ) Protein expression levels detected in ovarian protein extracts from Bactrian camels via iTRAQ LC-MS/MS. ( B ) Western blot analysis of PAK1, PRL, STAG2, TGFB2, CAMK2G, FST, NR5A1, and Wnt2b in ovarian protein extracts from Bactrian camels. ( C ) Integrated optical density analysis of the Western blots using ImageJ. Data are presented as the mean ± standard deviation; * p < 0.05, ** p < 0.01. ( D ) Venn diagram of the DEPs using multiple comparisons of the endocrine system, reproductive system and neural signal transduction system groups. ( E ) Immunofluorescence staining to localize the expression patterns of the DEPs in the ovarian samples.
Article Snippet: The membranes were blocked for 2 h in Tris–HCl buffer containing 5% ( w / v ) non-fat milk powder, then were incubated overnight at 4 °C with the following primary antibodies from mice or rabbits: ACTB (1:5000, Proteintech, China), CAMK2G (1:1000, Proteintech), FST (1:1000, Proteintech), NR5A1 (1:500, Proteintech), PAK1 (1:1000, Santa Cruz, USA), PRL (1:1000, Santa Cruz), STAG2 (1:1000, Santa Cruz), TGFB2 (1:500, Santa Cruz), Wnt2b (1:500, Santa Cruz), GAPDH (1:5000, Proteintech).
Techniques: Biomarker Discovery, Expressing, Multiplex sample analysis, Liquid Chromatography with Mass Spectroscopy, Western Blot, Standard Deviation, Transduction, Immunofluorescence, Staining
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